<h2 data-start="174" data-end="258">DTT (1,4-Dithiothreitol, AS-D0505): Essential Reducing Agent for Protein Analysis</h2>


<p data-start="260" data-end="372"><span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Astor Scientific offers <strong data-start="24" data-end="62">DTT (1,4-Dithiothreitol, AS-D0505), a high-purity reducing agent widely used in biochemical and molecular biology applications.</span> <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">This compound is crucial for maintaining the reduced state of thiol groups in proteins, facilitating accurate analysis and experimentation.</span>

<h3 data-start="374" data-end="396">Product Highlights</h3>
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<p data-start="400" data-end="416"><strong data-start="400" data-end="410">Purity: >99%

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<p data-start="419" data-end="477"><strong data-start="419" data-end="436">UV Absorbance: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out"><0.1 at 280 nm (1% solution)</span>

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<p data-start="480" data-end="532"><strong data-start="480" data-end="491">Storage: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Store at -20&deg;C</span>

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<p data-start="535" data-end="595"><strong data-start="535" data-end="554">Available Sizes: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">1g, 5g, 10g, 50g</span>

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<p data-start="598" data-end="687"><strong data-start="598" data-end="607">Price: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Starting at $36.00</span>

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<h3 data-start="771" data-end="787">Applications</h3>
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<p data-start="791" data-end="852"><strong data-start="791" data-end="811">Protein Analysis: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Reduces disulfide bonds in proteins, aiding in structural studies.</span>

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<p data-start="855" data-end="908"><strong data-start="855" data-end="867">SDS-PAGE: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Prepares protein samples by breaking disulfide linkages.</span>

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<p data-start="911" data-end="971"><strong data-start="911" data-end="930">Enzyme Activity: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Maintains the active form of enzymes sensitive to oxidation.</span>

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<p data-start="974" data-end="1081"><strong data-start="974" data-end="1001">Cross-Linking Reactions: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Breaks disulfide bridges in cross-linkers like N,N&prime;-bis(acryloyl)cystamine.</span>

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<h3 data-start="1083" data-end="1115">Advantages Over Alternatives</h3>
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<p data-start="1119" data-end="1174"><strong data-start="1119" data-end="1133">Less Toxic: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">DTT is less toxic and less pungent compared to 2-mercaptoethanol.</span>

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<p data-start="1177" data-end="1289"><strong data-start="1177" data-end="1209">Lower Concentration Required: <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Typically, a seven-fold lower concentration of DTT (100 mM) is needed than 2-mercaptoethanol (5% v/v, 700 mM).</span>

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<h3 data-start="1291" data-end="1323">Why Choose Astor Scientific?</h3>
<p data-start="1325" data-end="1483"><span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Astor Scientific provides high-quality reagents with exceptional customer service.</span> <span class="relative -mx-px my-[-0.2rem] rounded px-px py-[0.2rem] transition-colors duration-100 ease-in-out">Their platform offers a comprehensive selection of life science products, ensuring that researchers have access to the tools they need for their studies.</span>

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